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验证数据展示

  • WB analysis using 82950-1-RR

    WB analysis using 82950-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82950-1-RR (HEBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 82950-1-RR

    WB analysis of A549 using 82950-1-RR

    WB result of HEBP1 antibody (82950-1-RR; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-HEBP1 transfected A549 cells.

  • IHC staining of mouse liver using 82950-1-RR

    IHC staining of mouse liver using 82950-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 82950-1-RR (HEBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of U2OS using 82950-1-RR

    FC experiment of U2OS using 82950-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug HEBP1 Recombinant antibody (82950-1-RR, Clone:230156B6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82950-1-RR

    Affinity and Kinetic Characterization of 82950-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82950-1-RR against Human HEBP1 were performed. The affinity constant is 25.2 nM.

  • Featured Product
  • KD/KO验证

HEBP1 Recombinant antibody

HEBP1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 82950-1-RR

产品说明书

CloneNo. 230156B6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 750
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, L02 cells, HepG2 cells, rat liver tissue. mouse liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82950-1-RR targets HEBP1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HEBP1 fusion protein Ag10538 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heme binding protein 1
别名 Heme-binding protein 1, heme binding protein 1, HEBP 1, HEBP, HBP
计算分子量 189 aa, 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC016277
基因名称 HEBP1
Gene ID (NCBI) 50865
RRIDAB_3670693
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NRV9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HEBP1 antibody 82950-1-RRDownload protocol
IHC protocol for HEBP1 antibody 82950-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols