• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HEBP2 Polyclonal antibody

HEBP2 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12706-1-AP

Print datasheet

Synonyms

C6orf34, C6ORF34B, HEBP2, heme binding protein 2, KIAA1244, Placental protein 23, PP23, Protein SOUL, SOUL

验证数据展示

  • WB analysis of human brain using 12706-1-AP

    WB analysis of human brain using 12706-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12706-1-AP (HEBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 12706-1-AP

    IP experiment of HEK-293 using 12706-1-AP

    IP result of anti-HEBP2 (IP:12706-1-AP, 3ug; Detection:12706-1-AP 1:500) with HEK-293 cells lysate 2400ug.

经过测试的应用

Positive WB detected inhuman brain tissue, human kidney tissue
Positive IP detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12706-1-AP targets HEBP2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen HEBP2 fusion protein Ag3387 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heme binding protein 2
Synonyms C6orf34, C6ORF34B, HEBP2, heme binding protein 2, KIAA1244, Placental protein 23, PP23, Protein SOUL, SOUL
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC008205
Gene Symbol HEBP2
Gene ID (NCBI) 23593
RRIDAB_2232981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5Z4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEBP2(Heme-binding protein 2), also named SOUL and C6orf34, can promote mitochondrial permeability transition and facilitate necrotic cell death under different types of stress conditions (PMID: 17098234). It is a 23 kDa haem-binding protein that was first identified as the PP23 protein isolated from human full-term placentas. hEBP2 is essential for the proliferation of human cells and the repression of hEBP2 severely decreases the ability of EBNA1 and EBV-based plasmids to bind mitotic chromosomes (PMID: 15923612).

实验方案

Product Specific Protocols
WB protocol for HEBP2 antibody 12706-1-APDownload protocol
IP protocol for HEBP2 antibody 12706-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols