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HECTD2 Polyclonal antibody

HECTD2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25351-1-AP

Print datasheet

Synonyms

HECT domain containing 2, HECTD2

验证数据展示

  • WB analysis of mouse brain using 25351-1-AP

    WB analysis of mouse brain using 25351-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25351-1-AP (HECTD2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 25351-1-AP is HECTD2 Fusion Protein expressed in E. coli.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen HECTD2 fusion protein Ag18360 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HECT domain containing 2
Synonyms HECT domain containing 2, HECTD2
Calculated Molecular Weight 776 aa, 88 kDa
Observed Molecular Weight 88 kDa
GenBank Accession NumberBC040187
Gene Symbol HECTD2
Gene ID (NCBI) 143279
RRIDAB_2880040
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5U5R9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HECTD2(HECT domain-containing protein 2) may be an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for HECTD2 antibody 25351-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols