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验证数据展示

  • WB analysis using 32026-1-AP

    WB analysis using 32026-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32026-1-AP (HECW2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 32026-1-AP

    IF Staining of A549 using 32026-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HECW2 antibody (32026-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • IHC staining of mouse cerebellum using 32026-1-AP

    IHC staining of mouse cerebellum using 32026-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat cerebellum using 32026-1-AP

    IHC staining of rat cerebellum using 32026-1-AP

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 32026-1-AP (HECW2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HECW2 Polyclonal antibody

HECW2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 32026-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

NEDD4-like E3 ubiquitin-protein ligase 2, KIAA1301, HECT-type E3 ubiquitin transferase HECW2, HECT, C2 and WW domain-containing protein 2, EC:2.3.2.26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMDA-MB-468 cells, U-118 MG cells
Positive IHC detected inmouse cerebellum tissue, rat cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32026-1-AP targets HECW2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 HECW2 fusion protein Ag36316 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2
别名 NEDD4-like E3 ubiquitin-protein ligase 2, KIAA1301, HECT-type E3 ubiquitin transferase HECW2, HECT, C2 and WW domain-containing protein 2, EC:2.3.2.26
计算分子量176 kDa，1572aa
观测分子量240 kDa
GenBank蛋白编号XM_017004563.1
基因名称 HECW2
Gene ID (NCBI) 57520
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9P2P5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HECW2, also known as NEDL2 (Nedd4-like E3 ubiquitin-protein ligase 2), is a member of the HECT (Homologous to the E6-AP Carboxyl Terminus) family of E3 ubiquitin ligases. It is crucial in various pathophysiological processes, including cell proliferation, apoptosis, autophagy, immune response, and DNA damage repair (PMID: 39529070). HECW2 is known to stabilize and enhance the transcriptional activity of p73, a member of the p53 family of tumor suppressors, which promotes cell cycle arrest and apoptosis and has an essential role in neurodevelopment (PMID: 27389779). In cancer, HECW2 is a promising target for tumor therapy due to its role in regulating cell cycle progression and angiogenesis (PMID: 39529070). In neurodevelopmental disorders, variants in HECW2 have been reported to cause a disorder characterized by hypotonia, seizures, and impaired language (PMID: 34321324).

实验方案

Product Specific Protocols
WB protocol for HECW2 antibody 32026-1-APDownload protocol
IHC protocol for HECW2 antibody 32026-1-APDownload protocol
IF protocol for HECW2 antibody 32026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols