• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HEK 293 cells Polyclonal antibody

HEK 293 cells Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 27347-1-AP

Print datasheet

Synonyms

293, HEK 293 cells, HEK293

验证数据展示

  • IF Staining of HEK-293 using 27347-1-AP

    IF Staining of HEK-293 using 27347-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 27347-1-AP (HEK 293 cells antibody) at dilution of 1:1000 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27347-1-AP targets HEK 293 cells in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen Cell Lysate 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HEK 293 cells
Synonyms 293, HEK 293 cells, HEK293
Gene Symbol
Gene ID (NCBI)
RRIDAB_2880850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Embryonic Kidney 293 cells, also often referred to as HEK 293, 293 cells, or less precisely as HEK cells are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells were generated in early 70s by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex Van der Eb's laboratory in Leiden, Holland. This antibody is designed to detect the protein of the HEK293 cells.

实验方案

Product Specific Protocols
IF protocol for HEK 293 cells antibody 27347-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Biol Macromol

Western blotting analysis of proteins separated by agarose native gel electrophoresis.

Authors - Chiaki Sakuma
View Article