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HELB Polyclonal antibody

HELB Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 24700-1-AP

Print datasheet

Synonyms

DNA helicase B, hDHB, HELB, helicase (DNA) B

验证数据展示

  • WB analysis of Jurkat using 24700-1-AP

    WB analysis of Jurkat using 24700-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24700-1-AP (HELB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 24700-1-AP

    IF Staining of HeLa using 24700-1-AP

    Immunofluorescent analysis of HeLa cells using 24700-1-AP (HELB antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IP experiment of HEK-293 using 24700-1-AP

    IP experiment of HEK-293 using 24700-1-AP

    IP result of anti-HELB (IP:24700-1-AP, 4ug; Detection:24700-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24700-1-AP is HELB Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Tested Reactivity human
Immunogen HELB fusion protein Ag20335 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name helicase (DNA) B
Synonyms DNA helicase B, hDHB, HELB, helicase (DNA) B
Calculated Molecular Weight 1087 aa, 123 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC157019
Gene Symbol HELB
Gene ID (NCBI) 92797
RRIDAB_2879680
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NG08
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HELB antibody 24700-1-APDownload protocol
IF protocol for HELB antibody 24700-1-APDownload protocol
IP protocol for HELB antibody 24700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols