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HELZ Polyclonal antibody

HELZ Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26635-1-AP

Print datasheet

Synonyms

KIAA0054, HUMORF5, DRHC, DHRC

验证数据展示

  • IHC staining of human breast cancer using 26635-1-AP

    IHC staining of human breast cancer using 26635-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26635-1-AP (HELZ antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of HeLa using 26635-1-AP

    WB analysis of HeLa using 26635-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 26635-1-AP (HELZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 26635-1-AP

    IF Staining of HepG2 using 26635-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HELZ antibody (26635-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26635-1-AP targets HELZ in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen HELZ fusion protein Ag24326 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name helicase with zinc finger
Synonyms KIAA0054, HUMORF5, DRHC, DHRC
Calculated Molecular Weight 1942 aa, 219 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC130582
Gene Symbol HELZ
Gene ID (NCBI) 9931
RRIDAB_2880584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HELZ antibody 26635-1-APDownload protocol
IHC protocol for HELZ antibody 26635-1-APDownload protocol
IF protocol for HELZ antibody 26635-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols