• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

HERC4 Polyclonal antibody

HERC4 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 13691-1-AP

Print datasheet

Synonyms

Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4

验证数据展示

  • WB analysis of human brain using 13691-1-AP

    WB analysis of human brain using 13691-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 13691-1-AP

    WB analysis using 13691-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13691-1-AP (HERC4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 13691-1-AP

    IP experiment of HeLa using 13691-1-AP

    IP result of anti-HERC4 (IP:13691-1-AP, 4ug; Detection:13691-1-AP 1:800) with HeLa cells lysate 2000ug.

  • IF Staining of HeLa using 13691-1-AP

    IF Staining of HeLa using 13691-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using HERC4 antibody (13691-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, human testis tissue, human placenta tissue, mouse testis tissue, rat testis tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13691-1-AP targets HERC4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HERC4 fusion protein Ag4566 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hect domain and RLD 4
Synonyms Probable E3 ubiquitin-protein ligase HERC4, KIAA1593, HECT-type E3 ubiquitin transferase HERC4, hect domain and RLD 4, HECT domain and RCC1-like domain-containing protein 4
Calculated Molecular Weight 1049 aa, 118 kDa
Observed Molecular Weight 107 kDa
GenBank Accession NumberBC039600
Gene Symbol HERC4
Gene ID (NCBI) 26091
RRIDAB_10596480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5GLZ8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HERC4, also known as HECT and RLD domain containing E3 ubiquitin protein ligase 4, is a protein encoded by the HERC4 gene in humans. HERC4 is a novel member of HERC family. The mRNA of HERC4 has been found in all examined tissues, with its levels being significantly higher in the brain and testis than in the placenta and heart. (PMID: 28430527)

实验方案

Product Specific Protocols
WB protocol for HERC4 antibody 13691-1-APDownload protocol
IF protocol for HERC4 antibody 13691-1-APDownload protocol
IP protocol for HERC4 antibody 13691-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF,IP

Elife

SOX4 facilitates PGR protein stability and FOXO1 expression conducive for human endometrial decidualization.

Authors - Pinxiu Huang
View Article