验证数据展示
经过测试的应用
| Positive IP detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
22692-1-AP targets HERC5 in IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IP, ELISA Application Description |
| 文献引用应用 | IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | HERC5 fusion protein Ag18449 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | hect domain and RLD 5 |
| 别名 | E3 ISG15--protein ligase HERC5, E3 ISG15 protein ligase HERC5, Cyclin-E-binding protein 1, Cyclin E binding protein 1, CEBP1 |
| 计算分子量 | 1024 aa, 117 kDa |
| 观测分子量 | 117 kDa |
| GenBank蛋白编号 | BC140716 |
| 基因名称 | HERC5 |
| Gene ID (NCBI) | 51191 |
| RRID | AB_2879151 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen Affinity purified |
| UNIPROT ID | Q9UII4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for HERC5 antibody 22692-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Posttranslational ISGylation of NLRP3 by HERCs enzymes facilitates inflammasome activation in models of inflammation
| ||
Adv Sci (Weinh) Autophagy Deficiency Induced by SAT1 Potentiates Tumor Progression in Triple-Negative Breast Cancer |