验证数据展示
经过测试的应用
| Positive WB detected in | BxPC-3 cells, Tunicamycin treated HepG2 cells, HeLa cells, LNCaP cells, U2OS cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10813-1-AP targets HERPUD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | HERPUD1 fusion protein Ag1084 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 |
| 别名 | HERP, MMF, MIF1, Methyl methanesulfonate, KIAA0025 |
| 计算分子量 | 44 kDa |
| 观测分子量 | 54 kDa |
| GenBank蛋白编号 | BC008320 |
| 基因名称 | HERPUD1 |
| Gene ID (NCBI) | 9709 |
| RRID | AB_2248317 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15011 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HERPUD1 antibody 10813-1-AP | Download protocol |
| IHC protocol for HERPUD1 antibody 10813-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oncogene Small extracellular vesicles containing miR-30a-3p attenuate the migration and invasion of hepatocellular carcinoma by targeting SNAP23 gene. | ||
J Alzheimers Dis The Herp protein pathway is not involved in the pro-amyloidogenic effect of hyperhomocysteinemia. | ||
Biol Reprod The unfolded protein response contributes to preimplantation mouse embryo death in the DDK syndrome. | ||
PLoS One C/EBP-beta regulates endoplasmic reticulum stress-triggered cell death in mouse and human models. | ||
Exp Cell Res ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.
| ||
Mol Cell Biochem Phe27Cys polymorphism of the human delta opioid receptor predisposes cells to compromised calcium signaling. |