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HESX1 Polyclonal antibody

HESX1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 17927-1-AP

Print datasheet

Synonyms

ANF, hAnf, HESX homeobox 1, HESX1, Homeobox protein ANF, RPX

验证数据展示

  • IHC staining of mouse testis using 17927-1-AP

    IHC staining of mouse testis using 17927-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 17927-1-AP

    IHC staining of mouse testis using 17927-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17927-1-AP (HESX1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17927-1-AP targets HESX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HESX1 fusion protein Ag12348 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HESX homeobox 1
Synonyms ANF, hAnf, HESX homeobox 1, HESX1, Homeobox protein ANF, RPX
Calculated Molecular Weight 185 aa, 21 kDa
GenBank Accession NumberBC093979
Gene Symbol HESX1
Gene ID (NCBI) 8820
RRIDAB_2118120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBX0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HESX1 (Homeobox gene expressed in stem cells 1) is a homeobox transcriptional repressor that was initially isolated from murine embryonic stem cells (ESCs). Hesx1 is crucial for proper forebrain and pituitary development in both mice and humans, and inactivating mutations in HESX1 underlie septo-optic dysplasia.

实验方案

Product Specific Protocols
IHC protocol for HESX1 antibody 17927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols