验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells |
| Positive IF/ICC detected in | PC-3 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11317-1-AP targets HEXA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | HEXA fusion protein Ag1854 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hexosaminidase A (alpha polypeptide) |
| Synonyms | TSD, N-acetyl-beta-glucosaminidase subunit alpha, Hexosaminidase subunit A, EC:3.2.1.52, Beta-N-acetylhexosaminidase subunit alpha |
| Calculated Molecular Weight | 529 aa, 60 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC018927 |
| Gene Symbol | HEXA |
| Gene ID (NCBI) | 3073 |
| RRID | AB_2118133 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P06865 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-hexosaminidase subunit alpha (HEXA) is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HEXA antibody 11317-1-AP | Download protocol |
| IHC protocol for HEXA antibody 11317-1-AP | Download protocol |
| IF protocol for HEXA antibody 11317-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Immunol VAMP8 is essential in anaphylatoxin-induced degranulation, TNF-alpha secretion, peritonitis, and systemic inflammation. | ||
Front Oncol Microglia-Specific Expression of HEXA and HEXB Leads to Poor Prognosis in Glioblastoma Patients.
| ||
Proc Natl Acad Sci U S A A small-molecule drug inhibits autophagy gene expression through the central regulator TFEB | ||
Nat Commun Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents |
