验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells |
Positive IHC detected in | human kidney tissue, human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB |
Tested Reactivity | Human, mouse |
Cited Reactivity | human |
Immunogen | HEXA fusion protein Ag30808 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | hexosaminidase A (alpha polypeptide) |
Synonyms | HEXA, Hexosaminidase subunit A, TSD |
Calculated Molecular Weight | 529 aa, 60 kDa |
Observed Molecular Weight | 50-54 kDa, 60-67 kDa |
GenBank Accession Number | BC018927 |
Gene Symbol | HEXA |
Gene ID (NCBI) | 3073 |
RRID | AB_2918259 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P06865 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).
实验方案
Product Specific Protocols | |
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WB protocol for HEXA antibody 29259-1-AP | Download protocol |
IHC protocol for HEXA antibody 29259-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |