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验证数据展示

  • WB analysis using 29259-1-AP

    WB analysis using 29259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29259-1-AP

    IHC staining of human kidney using 29259-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HEXA Polyclonal antibody

HEXA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29259-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

HEXA, Hexosaminidase subunit A, TSD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen HEXA fusion protein Ag30808 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hexosaminidase A (alpha polypeptide)
Synonyms HEXA, Hexosaminidase subunit A, TSD
Calculated Molecular Weight 529 aa, 60 kDa
Observed Molecular Weight 50-54 kDa, 60-67 kDa
GenBank Accession NumberBC018927
Gene Symbol HEXA
Gene ID (NCBI) 3073
RRIDAB_2918259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06865
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

实验方案

Product Specific Protocols
WB protocol for HEXA antibody 29259-1-APDownload protocol
IHC protocol for HEXA antibody 29259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Proteome Res

Proteome and Glycoproteome Analyses Reveal Regulation of Protein Glycosylation Site-Specific Occupancy and Lysosomal Hydrolase Maturation by N-Glycan-Dependent ER-Quality Control

Authors - Jingru Chen
View Article