• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 29259-1-AP

    WB analysis using 29259-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29259-1-AP (HEXA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29259-1-AP

    IHC staining of human kidney using 29259-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29259-1-AP (HEXA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HEXA Polyclonal antibody

HEXA Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29259-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, IHC, ELISA

别名

HEXA, Hexosaminidase subunit A, TSD

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHEK-293 cells, NIH/3T3 cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman kidney tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29259-1-AP targets HEXA in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse
文献引用反应性human
免疫原 HEXA fusion protein Ag30808 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hexosaminidase A (alpha polypeptide)
别名 HEXA, Hexosaminidase subunit A, TSD
计算分子量 529 aa, 60 kDa
观测分子量 50-54 kDa, 60-67 kDa
GenBank蛋白编号BC018927
基因名称 HEXA
Gene ID (NCBI) 3073
RRIDAB_2918259
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06865
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta-hexosaminidase subunit alpha is involved in catalysis of degradation of the ganglioside GM2. Disfunction of beta-hexosaminidase leads to an accumulation of GM2 ganglioside in neurons and neurodegenerative disorders termed the GM2 gangliosidoses. The enzyme is composed of two subunits, alpha and beta. This gene encodes alpha subunit of beta-hexosaminidase. Western blot with this antibody raised against HEXA detected 60 kDa (precursor) and 54 kDa (mature) size bands (PMID: 27682588, PMID: 30341570).

实验方案

Product Specific Protocols
WB protocol for HEXA antibody 29259-1-APDownload protocol
IHC protocol for HEXA antibody 29259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Proteome Res

Proteome and Glycoproteome Analyses Reveal Regulation of Protein Glycosylation Site-Specific Occupancy and Lysosomal Hydrolase Maturation by N-Glycan-Dependent ER-Quality Control

Authors - Jingru Chen
View Article