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HEYL Polyclonal antibody

HEYL Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 15679-1-AP

Print datasheet

Synonyms

hHeyL, HEY3, Hairy-related transcription factor 3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33

验证数据展示

  • IF Staining of HeLa using 15679-1-AP

    IF Staining of HeLa using 15679-1-AP

    Immunofluorescent analysis of Hela cells, using HEYL antibody 15679-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • WB analysis of MDA-MB-453s using 15679-1-AP

    WB analysis of MDA-MB-453s using 15679-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 15679-1-AP (HEYL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15679-1-AP

    IP experiment of HeLa using 15679-1-AP

    IP result of anti-HEYL (IP:15679-1-AP, 4ug; Detection:15679-1-AP 1:300) with HeLa cells lysate 2600ug.

经过测试的应用

Positive WB detected inMDA-MB-453s cells, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

15679-1-AP targets HEYL in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HEYL fusion protein Ag8163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hairy/enhancer-of-split related with YRPW motif-like
Synonyms hHeyL, HEY3, Hairy-related transcription factor 3, Hairy/enhancer-of-split related with YRPW motif-like protein, Class B basic helix-loop-helix protein 33
Calculated Molecular Weight 328 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC006087
Gene Symbol HEYL
Gene ID (NCBI) 26508
RRIDAB_2878167
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NQ87
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HEYL, also named as Hairy/enhancer-of-split related with YRPW motif-like protein, is a 328 amino acid protein, which belongs to the HEY family. HEYL as downstream effector of Notch signaling may be required for cardiovascular development. HEYL represses transcription by the cardiac transcriptional activators GATA4 and GATA6.

实验方案

Product Specific Protocols
WB protocol for HEYL antibody 15679-1-APDownload protocol
IF protocol for HEYL antibody 15679-1-APDownload protocol
IP protocol for HEYL antibody 15679-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Adv Sci (Weinh)

Silencing of NOTCH3 Signaling in Meniscus Smooth Muscle Cells Inhibits Fibrosis and Exacerbates Degeneration in a HEYL-Dependent Manner

Authors - Hao Sun
View Article