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HGD Polyclonal antibody

HGD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16465-1-AP

Print datasheet

Synonyms

AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

验证数据展示

  • WB analysis using 16465-1-AP

    WB analysis using 16465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16465-1-AP

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16465-1-AP

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16465-1-AP targets HGD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HGD fusion protein Ag9544 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name homogentisate 1,2-dioxygenase (homogentisate oxidase)
Synonyms AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase
Calculated Molecular Weight 37 kDa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC020792
Gene Symbol HGD
Gene ID (NCBI) 3081
RRIDAB_2248397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93099
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

实验方案

Product Specific Protocols
WB protocol for HGD antibody 16465-1-APDownload protocol
IHC protocol for HGD antibody 16465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols