• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 16465-1-AP

    WB analysis using 16465-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16465-1-AP (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16465-1-AP

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human liver using 16465-1-AP

    IHC staining of human liver using 16465-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16465-1-AP (HGD antibody) at dilution of 1:100 (under 40x lens).

HGD Polyclonal antibody

HGD Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16465-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16465-1-AP targets HGD in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HGD fusion protein Ag9544 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 homogentisate 1,2-dioxygenase (homogentisate oxidase)
别名 AKU, HGD, HGO, Homogentisate 1,2 dioxygenase, Homogentisate oxygenase, Homogentisic acid oxidase, Homogentisicase
计算分子量 37 kDa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020792
基因名称 HGD
Gene ID (NCBI) 3081
RRIDAB_2248397
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ93099
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).

实验方案

Product Specific Protocols
WB protocol for HGD antibody 16465-1-APDownload protocol
IHC protocol for HGD antibody 16465-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols