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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    WB analysis using 84931-5-RR (same clone as 84931-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84931-5-RR (HGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84931-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84931-5-RR

    Affinity and Kinetic Characterization of 84931-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84931-5-RR against Human HGD were performed. The affinity constant is 1.38 nM.

HGD Recombinant antibody, PBS Only

HGD Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 84931-5-PBS

产品说明书

CloneNo. 242485E11

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, Indirect ELISA

别名

Homogentisate 1,2-dioxygenase, Homogentisate 1,2 dioxygenase, HGO, EC:1.13.11.5, AKU

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84931-5-PBS targets HGD in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原 HGD fusion protein Ag9544 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 homogentisate 1,2-dioxygenase (homogentisate oxidase)
别名 Homogentisate 1,2-dioxygenase, Homogentisate 1,2 dioxygenase, HGO, EC:1.13.11.5, AKU
计算分子量 37 kDa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC020792
基因名称 HGD
Gene ID (NCBI) 3081
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ93099
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Homogentisate1,2-dioxygenase (HGD), also named as HGO, is a mononuclear Fe(II)-dependent oxygenase that catalyzes the third step in the pathway for the catabolism of tyrosine, the conversion of homogentisate (HG) to maleylacetoacetate (MAA) and it can exsit as a dimer or trimer(PMID:14678794). HGD consists of a single type of subunit with no intermolecular disulfide bridges and requires Fe2+ as a cofactor(PMID: 7705358). Defects in HGD are the cause of alkaptonuria (AKU)(PMID:10594001).