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HHIP Polyclonal antibody

HHIP Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 11654-1-AP

Print datasheet

Synonyms

hedgehog interacting protein, HHIP, HIP

验证数据展示

  • WB analysis of HEK-293 using 11654-1-AP

    WB analysis of HEK-293 using 11654-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11654-1-AP

    WB analysis of HEK-293 using 11654-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11654-1-AP (HHIP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

11654-1-AP targets HHIP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen HHIP fusion protein Ag2241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hedgehog interacting protein
Synonyms hedgehog interacting protein, HHIP, HIP
Calculated Molecular Weight 700 aa, 79 kDa
GenBank Accession NumberBC025311
Gene Symbol HHIP
Gene ID (NCBI) 64399
RRIDAB_2232921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QV1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HHIP is a member of the hedgehog-interacting protein (HHIP) family. The hedgehog (HH) proteins are evolutionarily conserved protein, which are important morphogens for a wide range of developmental processes, including anteroposterior patterns of limbs and regulation of left-right asymmetry in embryonic development. Multiple cell-surface receptors are responsible for transducing and/or regulating HH signals. The HHIP encoded by this gene is a highly conserved, vertebrate-specific inhibitor of HH signaling. It interacts with all three HH family members, SHH, IHH and DHH. Two single nucleotide polymorphisms (SNPs) near this gene are significantly associated with risk of chronic obstructive pulmonary disease (COPD). A single nucleotide polymorphism in this gene is also strongly associated with human height.

实验方案

Product Specific Protocols
WB protocol for HHIP antibody 11654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Mol Cell Biol

Sequential stabilization of RNF220 by RLIM and ZC4H2 during cerebellum development and Shh-group medulloblastoma progression.

Authors - Yuwei Li
View Article
humanWB

Int Immunopharmacol

Wogonin inhibits the growth of HT144 melanoma via regulating hedgehog signaling-mediated inflammation and glycolysis.

Authors - Ling Li
View Article