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HIBCH Monoclonal antibody, PBS Only

HIBCH Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B7B8

Cat no : 68157-1-PBS

Print datasheet

Synonyms

HIB CoA hydrolase, HIBCH, HIBYL COA H

验证数据展示

  • IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    IF Staining of HepG2 using 68157-1-Ig (same clone as 68157-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIBCH antibody (68157-1-Ig, Clone: 1B7B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

  • WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    WB analysis using 68157-1-Ig (same clone as 68157-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68157-1-Ig (HIBCH antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68157-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.

产品信息

68157-1-PBS targets HIBCH in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen HIBCH fusion protein Ag6473 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name 3-hydroxyisobutyryl-Coenzyme A hydrolase
Synonyms HIB CoA hydrolase, HIBCH, HIBYL COA H
Calculated Molecular Weight 43 kDa
Observed Molecular Weight38 kDa
GenBank Accession NumberBC067822
Gene Symbol HIBCH
Gene ID (NCBI) 26275
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ6NVY1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).