CoraLite® Plus 488-conjugated HIBCH Monoclonal antibody
HIBCH Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse, rat, rabbit, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1B7B8
验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HIBCH Ready-To-Use IHC Kit. HIBCH primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68157-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68157 targets HIBCH in IF/ICC applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, mouse, rat, rabbit, pig |
| Immunogen | HIBCH fusion protein Ag6473 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | 3-hydroxyisobutyryl-Coenzyme A hydrolase |
| Synonyms | HIB CoA hydrolase, HIBCH, HIBYL COA H |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC067822 |
| Gene Symbol | HIBCH |
| Gene ID (NCBI) | 26275 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6NVY1 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIBCH belongs to the enoyl-CoA hydratase/isomerase family. HIBCH has two isoforms with MW 43 kDa and 38 kDa. It is highly expressed in the liver and kidney, also detected in the heart, muscle and brain (at protein level), but not detected in the lung. Hydrolyzes 3-hydroxyisobutyryl-CoA (HIBYL-CoA), a saline catabolite. Has high activity toward isobutyryl-CoA. Could be an isobutyryl-CoA dehydrogenase that functions in valine catabolism. Also hydrolyzes 3-hydroxypropanoyl-CoA (PMID: 8824301).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HIBCH antibody CL488-68157 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |