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HIC1 Polyclonal antibody

HIC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24949-1-AP

Print datasheet

Synonyms

hic 1, HIC1, hypermethylated in cancer 1, ZBTB29

验证数据展示

  • WB analysis of Jurkat using 24949-1-AP

    WB analysis of Jurkat using 24949-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 24949-1-AP

    IHC staining of human colon using 24949-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HIC1 antibody included
  • IHC staining of human testis using 24949-1-AP

    IHC staining of human testis using 24949-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24949-1-AP (HIC1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HIC1 antibody included

经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IHC detected inhuman testis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIC1 Ready-To-Use IHC Kit. HIC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24949-1-AP is HIC1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HIC1 fusion protein Ag21849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypermethylated in cancer 1
Synonyms hic 1, HIC1, hypermethylated in cancer 1, ZBTB29
Calculated Molecular Weight 714 aa, 75 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC157027
Gene Symbol HIC1
Gene ID (NCBI) 3090
RRIDAB_2879816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIC1 antibody 24949-1-APDownload protocol
IHC protocol for HIC1 antibody 24949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.

Authors - Huan Wang
View Article
humanWB,IHC

Cancer Cell Int

ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression.

Authors - Junqiang Liu
View Article
humanIHC

Front Immunol

Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity

Authors - Yanlin Wu
View Article