HIC1 Polyclonal antibody

HIC1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24949-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IHC detected inrat lung tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIC1 Ready-To-Use IHC Kit. HIC1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24949-1-AP targets HIC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 HIC1 fusion protein Ag21849 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hypermethylated in cancer 1
别名 Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1
计算分子量 714 aa, 75 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC157027
基因名称 HIC1
Gene ID (NCBI) 3090
RRIDAB_2879816
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14526
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIC1 antibody 24949-1-APDownload protocol
IHC protocol for HIC1 antibody 24949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.

Authors - Huan Wang
humanWB,IHC

Cancer Cell Int

ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression.

Authors - Junqiang Liu
humanIHC

Front Immunol

Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity

Authors - Yanlin Wu
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