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HIC1 Polyclonal antibody

HIC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24949-1-AP

Print datasheet

Synonyms

Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1

验证数据展示

  • WB analysis of Jurkat using 24949-1-AP

    WB analysis of Jurkat using 24949-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24949-1-AP (HIC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat lung using 24949-1-AP

    IHC staining of rat lung using 24949-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 24949-1-AP (HIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIC1 antibody included
  • IHC staining of rat stomach using 24949-1-AP

    IHC staining of rat stomach using 24949-1-AP

    Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 24949-1-AP (HIC1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIC1 antibody included

经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IHC detected inrat lung tissue, rat stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIC1 Ready-To-Use IHC Kit. HIC1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24949-1-AP targets HIC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen HIC1 fusion protein Ag21849 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypermethylated in cancer 1
Synonyms Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1
Calculated Molecular Weight 714 aa, 75 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC157027
Gene Symbol HIC1
Gene ID (NCBI) 3090
RRIDAB_2879816
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14526
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIC1 antibody 24949-1-APDownload protocol
IHC protocol for HIC1 antibody 24949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer.

Authors - Huan Wang
View Article
humanWB,IHC

Cancer Cell Int

ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression.

Authors - Junqiang Liu
View Article
humanIHC

Front Immunol

Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity

Authors - Yanlin Wu
View Article