验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, NIH/3T3 cells |
Positive IHC detected in | rat lung tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IHC | See 2 publications below |
产品信息
24949-1-AP targets HIC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | HIC1 fusion protein Ag21849 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | hypermethylated in cancer 1 |
别名 | Zinc finger and BTB domain-containing protein 29, ZBTB29, hypermethylated in cancer 1, Hic-1, hic 1 |
计算分子量 | 714 aa, 75 kDa |
观测分子量 | 65-70 kDa |
GenBank蛋白编号 | BC157027 |
基因名称 | HIC1 |
Gene ID (NCBI) | 3090 |
RRID | AB_2879816 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q14526 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for HIC1 antibody 24949-1-AP | Download protocol |
IHC protocol for HIC1 antibody 24949-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Cell Dev Biol Integrated Analysis of Ferroptosis-Related Biomarker Signatures to Improve the Diagnosis and Prognosis Prediction of Ovarian Cancer. | ||
Cancer Cell Int ZBTB7A, a miR-144-3p targeted gene, accelerates bladder cancer progression via downregulating HIC1 expression. | ||
Front Immunol Ferroptosis-related gene HIC1 in the prediction of the prognosis and immunotherapeutic efficacy with immunological activity |