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HIF2α/EPAS1 Monoclonal antibody, PBS Only

HIF2α/EPAS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F6B12

Cat no : 66731-1-PBS

Print datasheet

Synonyms

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

验证数据展示

  • WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

    WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

    untreated and cobalt chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

产品信息

66731-1-PBS targets HIF2α/EPAS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen HIF2α/EPAS1 fusion protein Ag24886 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name endothelial PAS domain protein 1
Synonyms bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC051338
Gene Symbol HIF2α
Gene ID (NCBI) 2034
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99814
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.