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验证数据展示

  • WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

    WB analysis of HeLa using 66731-1-Ig (same clone as 66731-1-PBS)

    untreated and cobalt chloride treated Hela cells were subjected to SDS PAGE followed by western blot with 66731-1-Ig (EPAS1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    IHC staining of human colon cancer using 66731-1-Ig (same clone as 66731-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66731-1-Ig (EPAS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66731-1-PBS in a different storage buffer formulation.

HIF2α/EPAS1 Monoclonal antibody, PBS Only

HIF2α/EPAS1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66731-1-PBS

产品说明书

CloneNo. 2F6B12

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, IHC, Indirect ELISA

别名

bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66731-1-PBS targets HIF2α/EPAS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 HIF2α/EPAS1 fusion protein Ag24886 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 endothelial PAS domain protein 1
别名 bHLHe73, ECYT4, EPAS 1, EPAS1, HIF 1 alpha like factor, HIF 2 alpha, HIF2 alpha, HIF2A, HIF-2A, HIF2α, HIF-2α, HLF, Member of PAS protein 2, MOP2, PASD2
计算分子量 96 kDa
观测分子量 120 kDa
GenBank蛋白编号BC051338
基因名称 HIF2α
Gene ID (NCBI) 2034
RRIDAB_2882081
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99814
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Endothelial PAS domain-containing protein 1 (EPAS1), also known as Hypoxia-inducible factor 2-alpha (HIF2-alpha,HIF2A), is a transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD. EPAS1 is expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.