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验证数据展示

  • WB analysis of A431 using 27650-1-AP

    WB analysis of A431 using 27650-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis using 27650-1-AP

    WB analysis using 27650-1-AP

    Cobalt Chloride treated HeLa cells were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis using 27650-1-AP

    WB analysis using 27650-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27650-1-AP (HIF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27650-1-AP

    IF Staining of HeLa using 27650-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Tunicamycin treated HeLa cells using HIF3A antibody (27650-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

HIF3A Polyclonal antibody

HIF3A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27650-1-AP
Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Synonyms

HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inCobalt Chloride treated HeLa cells, A431 cells
Positive IF/ICC detected inTunicamycin treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy HIF3A Ready-To-Use IHC Kit. HIF3A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27650-1-AP targets HIF3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen HIF3A fusion protein Ag26574 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hypoxia inducible factor 3, alpha subunit
Synonyms HIF3 alpha, HIF 3A4, HIF 3A2, HIF 3A, HIF 3 alpha
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberNM_022462
Gene Symbol HIF3A
Gene ID (NCBI) 64344
RRIDAB_2880939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIF3A, Hypoxia-inducible factor 3-alpha, acts as a transcriptional regulator in adaptive response to low oxygen tension (PMID:11573933, PMID:16126907, PMID:19694616, PMID:20416395, PMID:21069422). It functions as an inhibitor of angiogenesis in hypoxic cells of the cornea. HIF3A also plays a role in the development of the cardiorespiratory system. HIF3A was up-regulated by hypoxia (PMID:16775626). Multiple alternatively spliced transcript variants have been found.

实验方案

Product Specific Protocols
WB protocol for HIF3A antibody 27650-1-APDownload protocol
IF protocol for HIF3A antibody 27650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Commun

Identification of oleoylethanolamide as an endogenous ligand for HIF-3α.

Authors - Xiaotong Diao
View Article
humanWB

Mol Metab

Genome-wide association study of adipocyte lipolysis in the GENetics of adipocyte lipolysis (GENiAL) cohort.

Authors - Agné Kulyté
  • KD Validated
View Article
mouseWB

Front Cell Dev Biol

HIF3A Inhibition Triggers Browning of White Adipocytes via Metabolic Rewiring.

Authors - Francesca Cuomo
  • KD Validated
View Article
mouseWB

Toxicol Lett

Knockdown of long noncoding RNA MIAT attenuates cigarette smoke-induced airway remodeling by downregulating miR-29c-3p-HIF3A axis.

Authors - Wenchao Gu
View Article
humanIHC

ERJ Open Res

Dynamic analysis of gene signatures in the progression of COPD

Authors - Junchao Jiang
View Article
mouseIHC

Bioact Mater

Synergistic rheumatoid arthritis therapy by interrupting the detrimental feedback loop to orchestrate hypoxia M1 macrophage polarization using an enzyme-catalyzed nanoplatform

Authors - Dong Guo
View Article