验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, rat brain tissue, rat kidney tissue, HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21749-1-AP targets HIGD1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF, IP |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | HIGD1A fusion protein Ag14027 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | HIG1 hypoxia inducible domain family, member 1A |
别名 | RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A |
计算分子量 | 93 aa, 10 kDa |
观测分子量 | 10 kDa |
GenBank蛋白编号 | BC000601 |
基因名称 | HIGD1A |
Gene ID (NCBI) | 25994 |
RRID | AB_10858789 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y241 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.
实验方案
Product Specific Protocols | |
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WB protocol for HIGD1A antibody 21749-1-AP | Download protocol |
IF protocol for HIGD1A antibody 21749-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Proc Natl Acad Sci U S A Higd1a is a positive regulator of cytochrome c oxidase.
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Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
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PLoS One Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress. |