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HIGD1A Polyclonal antibody

HIGD1A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 21749-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (2)

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells, HeLa cells, rat brain tissue, rat kidney tissue, HepG2 cells
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21749-1-AP targets HIGD1A in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human
文献引用反应性human
免疫原 HIGD1A fusion protein Ag14027 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HIG1 hypoxia inducible domain family, member 1A
别名 RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A
计算分子量 93 aa, 10 kDa
观测分子量 10 kDa
GenBank蛋白编号BC000601
基因名称 HIGD1A
Gene ID (NCBI) 25994
RRIDAB_10858789
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y241
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

实验方案

Product Specific Protocols
WB protocol for HIGD1A antibody 21749-1-APDownload protocol
IF protocol for HIGD1A antibody 21749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF, IP

Proc Natl Acad Sci U S A

Higd1a is a positive regulator of cytochrome c oxidase.

Authors - Takaharu Hayashi
  • KD Validated
humanWB,IF,IP

Oncogene

DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.

Authors - Bin Chen
  • KD Validated
humanWB

Mol Cell Proteomics

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV

Authors - Daniella H Hock
  • KO Validated
humanWB

FASEB J

Higd1a improves respiratory function in the models of mitochondrial disorder.

Authors - Takemasa Nagao
humanWB

Mol Cell Proteomics

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.

Authors - Daniella H Hock
  • KO Validated
WB,IF

PLoS One

Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress.

Authors - Ameri Kurosh K
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