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HIGD1A Polyclonal antibody
HIGD1A Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293T cells, HeLa cells, rat brain tissue, rat kidney tissue, HepG2 cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 21749-1-AP is HIGD1A Fusion Protein expressed in E. coli.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, rat |
Cited Reactivity | human |
Immunogen | HIGD1A fusion protein Ag14027 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | HIG1 hypoxia inducible domain family, member 1A |
Synonyms | HIG1, HIG1 domain family member 1A, HIGD1A |
Calculated Molecular Weight | 93 aa, 10 kDa |
Observed Molecular Weight | 10 kDa |
GenBank Accession Number | BC000601 |
Gene Symbol | HIGD1A |
Gene ID (NCBI) | 25994 |
RRID | AB_10858789 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9Y241 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HIGD1A antibody 21749-1-AP | Download protocol |
IHC protocol for HIGD1A antibody 21749-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Proc Natl Acad Sci U S A Higd1a is a positive regulator of cytochrome c oxidase.
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Oncogene DNA damage-induced translocation of mitochondrial factor HIGD1A into the nucleus regulates homologous recombination and radio/chemo-sensitivity.
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV
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Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.
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PLoS One Nuclear localization of the mitochondrial factor HIGD1A during metabolic stress. |