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验证数据展示

  • IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

    IF Staining of U2OS using 68231-1-Ig (same clone as 68231-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HIGD1A antibody (68231-1-Ig, Clone: 3A8E2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

  • WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

    WB analysis using 68231-1-Ig (same clone as 68231-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68231-1-Ig (HIGD1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68231-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50454-2

    Cytometric bead array standard curve of MP50454-2

    Cytometric bead array standard curve of MP50454-2, HIGD1A Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68231-1-PBS. Detection antibody: 68231-4-PBS. Standard:Ag14027. Range: 0.391-100 ng/mL.

HIGD1A Monoclonal antibody, PBS Only (Capture)

HIGD1A Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 68231-1-PBS

产品说明书

CloneNo. 3A8E2

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68231-1-PBS targets HIGD1A as part of a matched antibody pair:

MP50454-2: 68231-1-PBS capture and 68231-4-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 HIGD1A fusion protein Ag14027 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 HIG1 hypoxia inducible domain family, member 1A
别名 RCF1 homolog A, Hypoxia-inducible gene 1 protein, HSPC010, HIG1 domain family member 1A, mitochondrial, HIG1 domain family member 1A
计算分子量 93 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC000601
基因名称 HIGD1A
Gene ID (NCBI) 25994
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y241
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (PMID:22342701). It is induced by hypoxia induction.