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HIGD1B Polyclonal antibody

HIGD1B Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 25367-1-AP

Print datasheet

Synonyms

Protein CLST 11240, CLST11240 15, CLST11240

验证数据展示

  • IHC staining of human colon using 25367-1-AP

    IHC staining of human colon using 25367-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25367-1-AP (HIGD1B Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of rat heart using 25367-1-AP

    IHC staining of rat heart using 25367-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 25367-1-AP (HIGD1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inrat heart tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25367-1-AP targets HIGD1B in IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity human
Immunogen HIGD1B fusion protein Ag21871 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HIG1 hypoxia inducible domain family, member 1B
Synonyms Protein CLST 11240, CLST11240 15, CLST11240
Calculated Molecular Weight 99 aa, 11 kDa
GenBank Accession NumberBC020667
Gene Symbol HIGD1B
Gene ID (NCBI) 51751
RRIDAB_2880047
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P298
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. HIG1 domain family member 1B (HIGD1B) is localized to the cell membrane whose expression is induced by hypoxia and glucose deprivation. (PMID: 34026765)

实验方案

Product Specific Protocols
IHC protocol for HIGD1B antibody 25367-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols