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  • Featured Product
  • KD/KO Validated

HINFP Polyclonal antibody, PBS Only

HINFP Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 10066-2-PBS

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Synonyms

验证数据展示

  • IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

    IF Staining of HeLa using 10066-2-AP (same clone as 10066-2-PBS)

    Immunofluorescent analysis of fixed Hela cells using 10066-2-AP (HINFP antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis of human liver using 10066-2-AP (same clone as 10066-2-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    WB analysis using 10066-2-AP (same clone as 10066-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10066-2-AP (HINFP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

  • IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP experiment of L02 using 10066-2-AP (same clone as 10066-2-PBS)

    IP result of anti-HINFP (IP:10066-2-AP, 4ug; Detection:10066-2-AP 1:2000) with L02 cells lysate 1040 ug. This data was developed using the same antibody clone with 10066-2-PBS in a different storage buffer formulation.

产品信息

10066-2-PBS targets HINFP in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen HINFP fusion protein Ag0115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone H4 transcription factor
Synonyms
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60-70 kDa
GenBank Accession NumberBC001073
Gene Symbol HINFP
Gene ID (NCBI) 25988
RRIDAB_2117412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQA5
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Methylation of CpG dinucleotides is the major epigenetic modification in higher eukaryotic genomes. MIZF interacts with MBD2, a methyl-CpG-binding protein playing a critical role in DNA methylation-mediated transcriptional repression. MIZF functions as a negative regulator of transcription by binding to MBD2 and recruiting histone deacetylase (HDAC)-containing complexes. MIZF mRNA is present in various human tissues and cell lines and expressed as a 60kd protein.