• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 17986-1-AP

    WB analysis using 17986-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17986-1-AP (HINT2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17986-1-AP

    WB analysis of HeLa using 17986-1-AP

    WB result of HINT2 antibody (17986-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT2 transfected HeLa cells.

  • IHC staining of human ovary cancer using 17986-1-AP

    IHC staining of human ovary cancer using 17986-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 17986-1-AP (HINT2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 17986-1-AP

    IF Staining of HeLa using 17986-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HINT2 antibody (17986-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

HINT2 Polyclonal antibody

HINT2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 17986-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Adenosine 5'-monophosphoramidase HINT2, EC:3.9.1.-, HINT 2, HINT 3, HINT-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17986-1-AP targets HINT2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 HINT2 fusion protein Ag12413 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidine triad nucleotide binding protein 2
别名 Adenosine 5'-monophosphoramidase HINT2, EC:3.9.1.-, HINT 2, HINT 3, HINT-2
计算分子量 163 aa, 17 kDa
观测分子量~17 kDa
GenBank蛋白编号BC047737
基因名称 HINT2
Gene ID (NCBI) 84681
RRIDAB_3669287
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BX68
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HINT2 antibody 17986-1-APDownload protocol
IHC protocol for HINT2 antibody 17986-1-APDownload protocol
IF protocol for HINT2 antibody 17986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols