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验证数据展示

  • WB analysis using 11366-1-AP

    WB analysis using 11366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11366-1-AP (HINT3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 11366-1-AP

    IHC staining of mouse liver using 11366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 11366-1-AP

    IHC staining of mouse liver using 11366-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11366-1-AP (HINT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HINT3 Polyclonal antibody

HINT3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11366-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

HINT 3, HINT3, HINT4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, human placenta tissue
Positive IHC detected inmouse liver tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11366-1-AP targets HINT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 HINT3 fusion protein Ag1912 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 histidine triad nucleotide binding protein 3
别名 HINT 3, HINT3, HINT4
计算分子量 182 aa, 20 kDa
观测分子量20-24 kDa
GenBank蛋白编号BC015732
基因名称 HINT3
Gene ID (NCBI) 135114
RRIDAB_3669127
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQE9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human Hint3 (hHint3) has been classified as a member of the histidine triad nucleotide (Hint) binding protein subfamily. HIT superfamily consists primarily of nucleoside phosphoramidases, dinucleotide hydrolases, and nucleotidyl transferases. While Hint1 is ubiquitously expressed by both eukaryotes and prokaryotes, Hint3 is found only in eukaryotes.

实验方案

Product Specific Protocols
WB protocol for HINT3 antibody 11366-1-APDownload protocol
IHC protocol for HINT3 antibody 11366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols