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验证数据展示

  • WB analysis using 22231-1-AP

    WB analysis using 22231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 22231-1-AP

    IF Staining of A549 using 22231-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IP experiment of HeLa using 22231-1-AP

    IP experiment of HeLa using 22231-1-AP

    IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug.

  • IHC staining of human colon cancer using 22231-1-AP

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human colon cancer using 22231-1-AP

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 22231-1-AP

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 22231-1-AP

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • Featured Product
  • KD/KO验证

HIP1 Polyclonal antibody

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22231-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

别名

HIP 1, HIP I, HIP1, ILWEQ

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inDU 145 cells, PC-3 cells, human brain tissue, A549 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, human colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22231-1-AP targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 HIP1 fusion protein Ag17576 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin interacting protein 1
别名 HIP 1, HIP I, HIP1, ILWEQ
计算分子量 1037 aa, 116 kDa
观测分子量 116 kDa
GenBank蛋白编号BC110545
基因名称 HIP1
Gene ID (NCBI) 3092
RRIDAB_2879041
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDO00291
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIP1 antibody 22231-1-APDownload protocol
IHC protocol for HIP1 antibody 22231-1-APDownload protocol
IF protocol for HIP1 antibody 22231-1-APDownload protocol
IP protocol for HIP1 antibody 22231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Acta Biochim Biophys Sin (Shanghai)

Y-box protein-1 modulates circSPECC1 to promote glioma tumorigenesis via miR-615-5p/HIP1/AKT axis

Authors - Ping Lan
View Article
humanWB,IHC

Oncol Lett

Abnormally high HIP1 expression is associated with metastatic behaviors and poor prognosis in ESCC.

Authors - Ying Sun
View Article
humanWB

FASEB J

MicroRNA-135a-3p regulates angiogenesis and tissue repair by targeting p38 signaling in endothelial cells.

Authors - Basak Icli
  • KD Validated
View Article