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HIP1-Specific Polyclonal antibody

HIP1-Specific Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19688-1-AP

Print datasheet

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

验证数据展示

  • WB analysis of HEK-293 using 19688-1-AP

    WB analysis of HEK-293 using 19688-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 19688-1-AP (HIP1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 19688-1-AP

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human prostate cancer using 19688-1-AP

    IHC staining of human prostate cancer using 19688-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 19688-1-AP (HIP1-Specific antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IP experiment of HEK-293 using 19688-1-AP

    IP experiment of HEK-293 using 19688-1-AP

    IP result of anti-HIP1-Specific (IP:19688-1-AP, 4ug; Detection:19688-1-AP 1:1000) with HEK-293 cells lysate 2400ug.

经过测试的应用

Positive WB detected inHEK-293 cells, mouse colon tissue, COLO 320 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19688-1-AP targets HIP1-Specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name huntingtin interacting protein 1
Synonyms HIP 1, HIP I, HIP1, ILWEQ
Calculated Molecular Weight 116 kDa
Observed Molecular Weight 116 kDa
GenBank Accession NumberNM_005338
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_10640443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
WB protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IHC protocol for HIP1-Specific antibody 19688-1-APDownload protocol
IP protocol for HIP1-Specific antibody 19688-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols