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HIP1R Polyclonal antibody

HIP1R Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16814-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, HeLa cells, mouse lung tissue, mouse heart tissue, human liver tissue, mouse kidney tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16814-1-AP targets HIP1R in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 HIP1R fusion protein Ag10353 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 huntingtin interacting protein 1 related
别名 HIP 12, HIP1 related protein, HIP12, HIP1R, HIP3, ILWEQ, KIAA0655
计算分子量 615 aa, 70 kDa, 120 kDa
观测分子量 120 kDa
GenBank蛋白编号BC067085
基因名称 HIP1R
Gene ID (NCBI) 9026
RRIDAB_2117572
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75146
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIP1R antibody 16814-1-APDownload protocol
IHC protocol for HIP1R antibody 16814-1-APDownload protocol
IP protocol for HIP1R antibody 16814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

Targeting Pin1 renders pancreatic cancer eradicable by synergizing with immunochemotherapy.

Authors - Kazuhiro Koikawa
  • KD Validated
humanWB

Nat Cell Biol

Acetylation-dependent regulation of PD-L1 nuclear translocation dictates the efficacy of anti-PD-1 immunotherapy.

Authors - Yang Gao
  • KO Validated
humanWB

Nat Biomed Eng

Inhibiting PD-L1 palmitoylation enhances T-cell immune responses against tumours.

Authors - Han Yao
humanWB,IP,IF

Nat Chem Biol

HIP1R targets PD-L1 to lysosomal degradation to alter T cell-mediated cytotoxicity.

Authors - Huanbin Wang
  • KD Validated
humanWB

Sci Adv

The Huntingtin-interacting protein SETD2/HYPB is an actin lysine methyltransferase.

Authors - Riyad N H Seervai
  • KD Validated
humanIHC

J Clin Med

HIP1R Expression and Its Association with PD-1 Pathway Blockade Response in Refractory Advanced NonSmall Cell Lung Cancer: A Gene Set Enrichment Analysis.

Authors - Young Wha Koh
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