- Featured Product
- KD/KO Validated
HIPK2 Polyclonal antibody
HIPK2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human renal cell carcinoma tissue, mouse heart tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HIPK2 Ready-To-Use IHC Kit. HIPK2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55408-1-AP targets HIPK2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC, IF, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | homeodomain interacting protein kinase 2 |
| Synonyms | PRO0593, Homeodomain-interacting protein kinase 2, hHIPk2, FLJ23711, EC:2.7.11.1 |
| Calculated Molecular Weight | 131 kDa |
| Observed Molecular Weight | 131 kDa, 101 kDa |
| GenBank Accession Number | NM_022740 |
| Gene Symbol | HIPK2 |
| Gene ID (NCBI) | 28996 |
| RRID | AB_2881323 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H2X6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIPK2 is a serine/threonine kinase participating in transcriptional regulation, growth control, and apoptosis. The kinase contributes to the regulation of remarkably diverse pathways such as p53 activation or Wnt signaling. There is very low levels of HIPK2 protein expression in several thyroid tumors and the combination of HIPK2 and Gal-3,would be helpful in ameliorating the preoperative recognition of thyroid cancer. HIPK2 may represent a new tumour suppressor gene for these types of cancers and may constitute a new potential promising diagnostic marker and therapeutic target.(PMID:21698151).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HIPK2 antibody 55408-1-AP | Download protocol |
| IHC protocol for HIPK2 antibody 55408-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Chem Biol Interact HIPK2 overexpression relieves hypoxia/reoxygenation-induced apoptosis and oxidative damage of cardiomyocytes through enhancement of the Nrf2/ARE signaling pathway.
| ||
Cell Prolif Homeodomain-interacting protein kinase 2 suppresses proliferation and aerobic glycolysis via ERK/cMyc axis in pancreatic cancer. | ||
Int J Biol Macromol Exosomal miR-1229 derived from colorectal cancer cells promotes angiogenesis by targeting HIPK2. | ||
Cell Res Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine synthesis to p53 activation to control cell fate |