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Host
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Species Reactivity
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验证数据展示

  • IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    IF Staining of HeLa using 83194-5-RR (same clone as 83194-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIRA antibody (83194-5-RR, Clone: 240006E5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 83194-5-RR (same clone as 83194-5-PBS)

    FC experiment of K-562 using 83194-5-RR (same clone as 83194-5-PBS)

    1x10^6 K-562 cells were intracellularly stained with 0.25 ug HIRA Recombinant Antibody (83194-5-RR, Clone:240006E5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83194-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83194-5-RR

    Affinity and Kinetic Characterization of 83194-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83194-5-RR against Human HIRA were performed. The affinity constant is 7.91 nM.

HIRA Recombinant antibody, PBS Only

HIRA Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83194-5-PBS

产品说明书

CloneNo. 240006E5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

Protein HIRA, HIRA/HIR, HIR, 240006E5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83194-5-PBS targets HIRA in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 HIRA fusion protein Ag34439 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 HIR histone cell cycle regulation defective homolog A (S. cerevisiae)
别名 Protein HIRA, HIRA/HIR, HIR, 240006E5
计算分子量 112 kDa
GenBank蛋白编号NM_003325
基因名称 HIRA
Gene ID (NCBI) 7290
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP54198
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C