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HIRIP3 Polyclonal antibody

HIRIP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14992-1-AP

Print datasheet

Synonyms

HIRA interacting protein 3, HIRIP3

验证数据展示

  • WB analysis of mouse skeletal muscle using 14992-1-AP

    WB analysis of mouse skeletal muscle using 14992-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14992-1-AP (HIRIP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human ovary using 14992-1-AP

    IHC staining of human ovary using 14992-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14992-1-AP (HIRIP3 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, human heart tissue, human skeletal muscle tissue
Positive IHC detected inhuman ovary tissue, human heart tissue, human kidney tissue, human placenta tissue, human skin tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14992-1-AP targets HIRIP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen HIRIP3 fusion protein Ag6978 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name HIRA interacting protein 3
Synonyms HIRA interacting protein 3, HIRIP3
Calculated Molecular Weight 62 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC000588
Gene Symbol HIRIP3
Gene ID (NCBI) 8479
RRIDAB_2117876
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BW71
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The HIRIP3 protein had been identified from its interaction with the HIRA histone chaperone. It may play a role in chromatin function and histone metabolism via its interaction with HIRA and histones.

实验方案

Product Specific Protocols
WB protocol for HIRIP3 antibody 14992-1-APDownload protocol
IHC protocol for HIRIP3 antibody 14992-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols