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PPIP5K1 Polyclonal antibody

PPIP5K1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14331-1-AP

Print datasheet

Synonyms

HISPPD2A, EC:2.7.4.24, InsP6 and PP-IP5 kinase 1, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1, hsVIP1

验证数据展示

  • WB analysis using 14331-1-AP

    WB analysis using 14331-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14331-1-AP (PPIP5K1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 14331-1-AP

    IHC staining of mouse skeletal muscle using 14331-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14331-1-AP (PPIP5K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-251 using 14331-1-AP

    IF Staining of U-251 using 14331-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PPIP5K1 antibody (14331-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-251 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14331-1-AP targets PPIP5K1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen PPIP5K1 fusion protein Ag5614 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histidine acid phosphatase domain containing 2A
Synonyms HISPPD2A, EC:2.7.4.24, InsP6 and PP-IP5 kinase 1, Inositol hexakisphosphate and diphosphoinositol-pentakisphosphate kinase 1, hsVIP1
Calculated Molecular Weight 160 kDa
Observed Molecular Weight160 kDa
GenBank Accession NumberBC050263
Gene Symbol HISPPD2A
Gene ID (NCBI) 9677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6PFW1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653).

实验方案

Product Specific Protocols
WB protocol for PPIP5K1 antibody 14331-1-APDownload protocol
IHC protocol for PPIP5K1 antibody 14331-1-APDownload protocol
IF protocol for PPIP5K1 antibody 14331-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols