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Histone H1.5 Polyclonal antibody

Histone H1.5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 18093-1-AP

Print datasheet

Synonyms

HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5

验证数据展示

  • WB analysis using 18093-1-AP

    WB analysis using 18093-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18093-1-AP (Histone H1.5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 18093-1-AP

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IHC staining of mouse brain using 18093-1-AP

    IHC staining of mouse brain using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included
  • IF Staining of SH-SY5Y using 18093-1-AP

    IF Staining of SH-SY5Y using 18093-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Histone H1.5 antibody (18093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human colon using 18093-1-AP

    IHC staining of human colon using 18093-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18093-1-AP (Histone H1.5 antibody) at dilution of 1:6000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIST1H1B antibody included

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, LnCaP cells
Positive IHC detected inmouse brain tissue, human colon tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HIST1H1B Ready-To-Use IHC Kit. HIST1H1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:30000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18093-1-AP targets Histone H1.5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Histone H1.5 fusion protein Ag12818 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H1b
Synonyms HIST1H1B, histone cluster 1, H1b, Histone, H1F5, H1-5
Calculated Molecular Weight 226 aa, 23 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC101581
Gene Symbol HIST1H1B
Gene ID (NCBI) 3009
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP16401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Histone H1.5, also known as Histone Cluster 1 H1 Family Member B (HIST1H1B), is a member of the Histone H1/H5 protein family. Histone H1.5 gene has been found to be frequently mutated in colorectal cancer and follicular lymphomas. HIST1H1B overexpression contributes to tumor aggressiveness. Histone H1.5 expression is upregulated in breast cancer and predicts poor prognosis(PMID: 34746019).

实验方案

Product Specific Protocols
WB protocol for Histone H1.5 antibody 18093-1-APDownload protocol
IHC protocol for Histone H1.5 antibody 18093-1-APDownload protocol
IF protocol for Histone H1.5 antibody 18093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols