验证数据展示

WB analysis of MCF-7 using 19649-1-AP
WB result of Histone H1.2 antibody (19649-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-Histone H1.2 transfected MCF-7 cells.
IF Staining of HeLa using 19649-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
WB analysis of L02 using 19649-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 19649-1-AP (HIST1H1C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 19649-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor using 19649-1-AP (HIST1H1C antibody) at dilution of 1:100 (under 40x lens).
IF Staining of HepG2 using 19649-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H1.2 antibody (19649-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).
IP experiment of HeLa using 19649-1-AP
IP result of anti-Histone H1.2 (IP:19649-1-AP, 3ug; Detection:19649-1-AP 1:500) with HeLa cells lysate 1600ug.
IHC staining of human normal colon using 19649-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 19649-1-AP (Histone H1.2 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO验证

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 19649-1-AP

产品说明书

引用文献(28)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

别名

Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2

缓冲液配方: PBS and Azide

PBS and Azide

偶联物: Unconjugated

Unconjugated

规格:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (28)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	L02 cells, human testis tissue, Jurkat cells, MCF-7 cells, A375 cells, mouse thymus tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human ovary tumor tissue, human normal colon **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells, HepG2 cells

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:600
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 23 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below
ChIP	See 1 publications below

产品信息

19649-1-AP targets Histone H1.2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用	WB, IHC, IF, IP, ChIP
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, camelus bactrianus
免疫原	Histone H1.2 fusion protein Ag7503 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	histone cluster 1, H1c
别名	Histone H1, histone cluster 1, H1c, HIST1H1C, H1F2, H1-2
计算分子量	21 kDa
观测分子量	32-33 kDa
GenBank蛋白编号	BC002649
基因名称	Histone H1.2
Gene ID (NCBI)	3006
RRID	AB_10694432
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P16403
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Histone H1 regulates chromatin competency through its high affinity binding to linker DNA as a structural component. It acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. The calculated molecular weight of Histone H1.2 is 21 kDa, but modified Histone H1.2 is about 32 kDa. (PMID: 18258596)

实验方案

Product Specific Protocols
WB protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
IHC protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
IF protocol for Histone H1.2 antibody 19649-1-AP	Download protocol
IP protocol for Histone H1.2 antibody 19649-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Nat Neurosci Genome-wide distribution of linker histone H1.0 is independent of MeCP2. Authors - Aya Ito-Ishida View Article
mouse	WB	Nat Commun Histone functions as a cell-surface receptor for AGEs. Authors - Masanori Itakura View Article
human	WB	Oncogene Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells. Authors - Mengyi Lao View Article
human	WB	Oxid Med Cell Longev The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress. Authors - Jun Tian View Article
human	WB	J Neuroinflammation New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis. Authors - Wen-Tong Liu View Article
human	IF	Front Immunol HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3. Authors - Xiaokun Liu View Article

验证数据展示

WB analysis of MCF-7 using 19649-1-AP

IF Staining of HeLa using 19649-1-AP

WB analysis of L02 using 19649-1-AP

IHC staining of human ovary tumor using 19649-1-AP

IF Staining of HepG2 using 19649-1-AP

IP experiment of HeLa using 19649-1-AP

IHC staining of human normal colon using 19649-1-AP

Histone H1.2 Polyclonal antibody

Histone H1.2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

Histone functions as a cell-surface receptor for AGEs.

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

The Formation of Melanocyte Apoptotic Bodies in Vitiligo and the Relocation of Vitiligo Autoantigens under Oxidative Stress.

New insights into meningitic Escherichia coli infection of brain microvascular endothelial cells from quantitative proteomics analysis.

HIST1H1C Regulates Interferon-β and Inhibits Influenza Virus Replication by Interacting with IRF3.