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HIST1H2AC Polyclonal antibody

HIST1H2AC Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15953-1-AP

Print datasheet

Synonyms

H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL

验证数据展示

  • WB analysis of mouse liver using 15953-1-AP

    WB analysis of mouse liver using 15953-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15953-1-AP (HIST1H2AC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 15953-1-AP

    IHC staining of human skin cancer using 15953-1-AP

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 15953-1-AP (HIST1H2AC antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, rat liver tissue
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15953-1-AP targets HIST1H2AC in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen HIST1H2AC fusion protein Ag8663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H2ac
Synonyms H2AC6, H2A/l, Histone H2A, histone cluster 1, H2ac, H2AFL
Calculated Molecular Weight 130 aa, 14 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC017379
Gene Symbol HIST1H2AC
Gene ID (NCBI) 8334
RRIDAB_2878199
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ93077
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIST1H2AC antibody 15953-1-APDownload protocol
IHC protocol for HIST1H2AC antibody 15953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Biology (Basel)

Identification of NRAS Downstream Genes with CRISPR Activation Screening

Authors - Akiya Tatsumi
View Article