验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells |
| Positive ELISA detected in | peptide |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:32000-1:128000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29202-1-AP targets Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone cluster 1, H3a |
| 别名 | Di-Methyl-Histone-H3, Di-Methyl-Histone H3, Di-Methyl Histone-H3 Lys36, Di-Methyl Histone H3 Lys36, Di-Methyl Histone H3 (Lys36) |
| 观测分子量 | 15-18 kDa |
| GenBank蛋白编号 | BC066245 |
| 基因名称 | HIST1H3A |
| Gene ID (NCBI) | 8350 |
| RRID | AB_2918247 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P68431 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
