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Di-Methyl-Histone H3 (Lys36) Polyclonal antibody

Di-Methyl-Histone H3 (Lys36) Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29202-1-AP

Print datasheet

Synonyms

H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

验证数据展示

  • WB analysis using 29202-1-AP

    WB analysis using 29202-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29202-1-AP (HIST1H3A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • ELISA experiment of peptide using 29202-1-AP

    ELISA experiment of peptide using 29202-1-AP

    Di-Methyl-Histone H3(Lys36) antibody (29202-1-AP) tested by ELISA. HIST1H3A(31-43aa), HIST1H3AMe1(31-43aa), HIST1H3AMe2(31-43aa), HIST1H3AMe3(31-43aa) were coated onto microtiter plates at 0.15 µg/well and then incubated with a dilution series of HIST1H3AMe2 antibody (29202-1-AP). Bound antibodies were detected with HRP conjugated anti-rabbit IgG followed by incubation with HRP substrate and then measuring the resulting absorbance at 450 nm.

经过测试的应用

Positive WB detected inC2C12 cells, HEK-293T cells, THP-1 cells, HeLa cells, HepG2 cells, HSC-T6 cells
Positive ELISA detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:32000-1:128000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29202-1-AP targets Di-Methyl-Histone H3 (Lys36) in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 1, H3a
Synonyms H3/A, H3FA, H3FB, H3FC, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3C, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, histone cluster 1, H3a, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
Observed Molecular Weight15-18 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_2918247
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones, including H1/H5 (linker histones), H2, H3, and H4 (core histones), are nucleic proteins which interact with DNA to form the nucleosomes and play important roles in gene regulation and DNA replication. Histone proteins are highly post-translationally modified while Histone H3 is the most extensively modified.The NSD family histone methyltransferases, including NSD1, NSD2 and NSD3, play crucial roles in chromatin regulation and are implicated in oncogenesis (PMID: 33361816).NSD enzymes exhibit an auto-inhibitory state that is relieved by nucleosome engagement, allowing for H3K36 di-methylation catalysis (PMID:22099308).

实验方案

Product Specific Protocols
WB protocol for Di-Methyl-Histone H3 (Lys36) antibody 29202-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols