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HIST2H2AA4 Polyclonal antibody

HIST2H2AA4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15302-1-AP

Print datasheet

Synonyms

H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o

验证数据展示

  • WB analysis of L02 using 15302-1-AP

    WB analysis of L02 using 15302-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 15302-1-AP

    IHC staining of human liver cancer using 15302-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15302-1-AP (HIST2H2AA4 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells, Jurkat cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15302-1-AP targets HIST2H2AA4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen HIST2H2AA4 fusion protein Ag7560 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 2, H2aa4
Synonyms H2A/R, H2AFO, HIST2H2AA, HIST2H2AA3, HIST2H2AA4, histone, histone cluster 2, H2aa4, Histone H2A type 2 A, Histone H2A.2, Histone H2A/o
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC001629
Gene Symbol HIST2H2AA4
Gene ID (NCBI) 723790
RRIDAB_2878124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6FI13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
IHC protocol for HIST2H2AA4 antibody 15302-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Physiol Biochem

Effect of Type I Diabetes on the Proteome of Mouse Oocytes.

Authors - Guangjian Jiang
View Article