Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15857-1-AP

Print datasheet

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q



经过测试的应用

Positive WB detected inHEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inN/A

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15857-1-AP targets Histone H2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IP, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, bovine
Immunogen Histone H2B fusion protein Ag7811 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 2, H2be
Synonyms GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q
Calculated Molecular Weight 14 kDa
Observed Molecular Weight14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
RRIDAB_10664929
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Histone H2B antibody 15857-1-APDownload protocol
IHC protocol for Histone H2B antibody 15857-1-APDownload protocol
IP protocol for Histone H2B antibody 15857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Nanotechnol

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Authors - Tanmoy Saha
humanIF

Nat Biomed Eng

Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues

Authors - Su-Jin Heo
humanWB

Oncogene

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Authors - Laura Monteonofrio
humanWB

Elife

Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.

Authors - William F Richter
humanWB

Mol Cell Proteomics

Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

Authors - Rui Sun
humanWB

Cell Discov

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Authors - Jialun Li