验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, fetal human brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10445-1-AP targets Histone H2A type 3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IP, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | Histone H2A type 3 fusion protein Ag0374 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | histone cluster 3, H2a |
| Synonyms | HIST3H2A, histone, histone cluster 3, H2a, Histone H2A, Histone H2A type 3 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-18 kDa |
| GenBank Accession Number | BC001193 |
| Gene Symbol | Histone H2A type 3 |
| Gene ID (NCBI) | 92815 |
| RRID | AB_2116701 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7L7L0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone H2A type 3, it is expected to be located in the nucleus, and the protein is enriched in the brain tissue. Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. The molecular weight of Histone H2A type 3 is 14 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone H2A type 3 antibody 10445-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res H2A.Z.1 crosstalk with H3K56-acetylation controls gliogenesis through the transcription of folate receptor.
| ||
Nucleic Acids Res Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder. | ||
EMBO Rep MacroH2A1.2 deficiency leads to neural stem cell differentiation defects and autism-like behaviors. | ||
BMC Cancer HIST3H2A promotes the progression of prostate cancer through inhibiting cell necroptosis |
