验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, mouse brain tissue, mouse testis tissue |
Planning an IHC experiment? We recommend our IHCeasy Histone H3.4 Ready-To-Use IHC Kit. Histone H3.4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18064-1-AP targets Histone H3.4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Histone H3.4 fusion protein Ag12671 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | histone cluster 3, H3 |
| 别名 | H3.4, H3/g, H3/t, H3FT, H3t, HIST3H3, histone cluster 3, H3, Histone H3.1t, Histone H3.4 |
| 计算分子量 | 136 aa, 16 kDa |
| 观测分子量 | 16 kDa |
| GenBank蛋白编号 | BC101837 |
| 基因名称 | Histone H3.4 |
| Gene ID (NCBI) | 8290 |
| RRID | AB_3669290 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16695 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone H3.4 antibody 18064-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |