• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Histone H3.4 Polyclonal antibody

Histone H3.4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 18064-1-AP

Print datasheet

Synonyms

H3.4, H3/g, H3/t, H3FT, H3t, HIST3H3, histone cluster 3, H3, Histone H3.1t, Histone H3.4

验证数据展示

  • WB analysis using 18064-1-AP

    WB analysis using 18064-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18064-1-AP (Histone H3.4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA431 cells, mouse brain tissue, mouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy Histone H3.4 Ready-To-Use IHC Kit. Histone H3.4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18064-1-AP targets Histone H3.4 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Histone H3.4 fusion protein Ag12671 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name histone cluster 3, H3
Synonyms H3.4, H3/g, H3/t, H3FT, H3t, HIST3H3, histone cluster 3, H3, Histone H3.1t, Histone H3.4
Calculated Molecular Weight 136 aa, 16 kDa
Observed Molecular Weight16 kDa
GenBank Accession NumberBC101837
Gene Symbol Histone H3.4
Gene ID (NCBI) 8290
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16695
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIST3H3, also known as H3.1t and H3.4, is a histone H3 protein variant that bears the H3.1 variant hallmark cysteine 96 but includes three extra amino acid substitutions. H3.1t was found to be expressed in primary human spermatocytes at the pachytene stage, similar to the human testis-specific histone H1t variant, where it appears therefore to take part in the process of spermatogenesis (PMID: 21782046).

实验方案

Product Specific Protocols
WB protocol for Histone H3.4 antibody 18064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols