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验证数据展示

  • WB analysis using 68995-1-Ig

    WB analysis using 68995-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68995-1-Ig (HJURP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

HJURP Monoclonal antibody

HJURP Monoclonal Antibody for WB, ELISA
Cat No. 68995-1-Ig

Print datasheet

CloneNo. 1F8C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Synonyms

Up regulated in lung cancer 9, hFLEG1, FLEG1, Fetal liver-expressing gene 1 protein, FAKTS

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inPANC-1 cells, SW 1990 cells, Saos2 cells, U2OS cells, A549 cells, HeLa cells, MDA-MB-468 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68995-1-Ig targets HJURP in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen HJURP fusion protein Ag34571 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Holliday junction recognition protein
Synonyms Up regulated in lung cancer 9, hFLEG1, FLEG1, Fetal liver-expressing gene 1 protein, FAKTS
Calculated Molecular Weight 86 kDa
Observed Molecular Weight85-90 kDa
GenBank Accession NumberBC001940
Gene Symbol HJURP
Gene ID (NCBI) 55355
RRIDAB_3670458
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ8NCD3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Holliday Junction Recognition Protein (HJURP, also known as hFLEG1) is a centromeric protein with a pivotal role in the incorporation and maintenance of histone H3-like variant Centromere Protein-A (CENP-A) in centromeres. It is a specific chaperone for CENP-A and it integrates newly synthesized CENP-A molecules and nucleosomes at replicated centromeres. (PMID: 28819432, PMID: 31492860)

实验方案

Product Specific Protocols
WB protocol for HJURP antibody 68995-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols