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验证数据展示

  • WB analysis using 55383-1-AP

    WB analysis using 55383-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55383-1-AP (HLA-A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 55383-1-AP

    IHC staining of human tonsillitis using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HLA-A antibody included
  • IHC staining of human tonsillitis using 55383-1-AP

    IHC staining of human tonsillitis using 55383-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 55383-1-AP (HLA-A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HLA-A antibody included

HLA-A Polyclonal antibody

HLA-A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55383-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA and More (1)

别名

Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA class I ABC, HLA class I (HLA-A), HLA class I

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 500 ug/ml

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经过测试的应用

Positive WB detected inHepG2 cells, A431 cells
Positive IHC detected inhuman tonsillitis tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HLA-A Ready-To-Use IHC Kit. HLA-A primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55383-1-AP targets HLA-A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, A
别名 Human leukocyte antigen A, HLA class I histocompatibility antigen, A alpha chain, HLA class I ABC, HLA class I (HLA-A), HLA class I
计算分子量 41 kDa
观测分子量 41-44 kDa
GenBank蛋白编号NM_002116
基因名称 HLA-A
Gene ID (NCBI) 3105
RRIDAB_10888639
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04439
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. (PMID: 667938; 3375250)

实验方案

Product Specific Protocols
WB protocol for HLA-A antibody 55383-1-APDownload protocol
IHC protocol for HLA-A antibody 55383-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Virol

Two Functional Variants of AP-1 Complexes Composed of either γ2 or γ1 Subunits Are Independently Required for Major Histocompatibility Complex Class I Downregulation by HIV-1 Nef.

Authors - Lucas A Tavares
View Article
humanIHC

Front Pharmacol

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis

Authors - Yanling Wang
View Article