HLA class I (HLA-B) Polyclonal antibody
HLA class I (HLA-B) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HepG2 cells, Jurkat cells, MCF-7 cells, HeLa cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human spleen tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human stomach cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
17260-1-AP targets HLA class I (HLA-B) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | HLA class I (HLA-B) fusion protein Ag10947 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | major histocompatibility complex, class I, B |
Synonyms | AS, HLA B, HLA B 7301, HLA B73, HLA class I (HLA-B), HLAB, HLA-B, HLAC, MHC class I antigen B*7, SPDA1 |
Calculated Molecular Weight | 362 aa, 40 kDa |
Observed Molecular Weight | 40-45 kDa |
GenBank Accession Number | BC013187 |
Gene Symbol | HLA class I (HLA-B) |
Gene ID (NCBI) | 3106 |
RRID | AB_1939610 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P01889 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
IHC protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
IF protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
IP protocol for HLA class I (HLA-B) antibody 17260-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Gut Single-cell RNA sequencing reveals intrahepatic and peripheral immune characteristics related to disease phases in HBV-infected patients. | ||
Elife Cell-surface tethered promiscuous biotinylators enable comparative small-scale surface proteomic analysis of human extracellular vesicles and cells. | ||
Cell Death Dis Exosomal annexin A6 induces gemcitabine resistance by inhibiting ubiquitination and degradation of EGFR in triple-negative breast cancer. | ||
bioRxiv Secreted HLA Fc-Fusion Profiles Immunopeptidome in Hypoxic PDAC and Cellular Senescence | ||
PNAS Nexus Secreted HLA-Fc fusion profiles immunopeptidome in hypoxic PDAC and cellular senescence |