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验证数据展示

  • WB analysis using 15777-1-AP

    WB analysis using 15777-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 15777-1-AP

    IP experiment of Jurkat using 15777-1-AP

    IP result of anti-HLA class I (HLA-C) (IP:15777-1-AP, 4ug; Detection:15777-1-AP 1:1000) with Jurkat cells lysate 2400ug.

  • IF Staining of HeLa using 15777-1-AP

    IF Staining of HeLa using 15777-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HLA class I (HLA-C) antibody (15777-1-AP) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • IHC staining of human tonsillitis using 15777-1-AP

    IHC staining of human tonsillitis using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 15777-1-AP

    IHC staining of human tonsillitis using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 15777-1-AP

    IHC staining of human tonsillitis using 15777-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15777-1-AP (HLA class I (HLA-C) antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HLA class I (HLA-C) Polyclonal antibody

HLA class I (HLA-C) Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15777-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

别名

HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15777-1-AP targets HLA class I (HLA-C) in WB, IHC, IF/ICC, IP, ELISA, Cell treatment applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, Cell treatment
经测试反应性 human
文献引用反应性human
免疫原 HLA class I (HLA-C) fusion protein Ag8461 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, C
别名 HLA-C, HLAC, HLA JY3, HLA Cw12, HLA Cw
计算分子量 366 aa, 41 kDa
观测分子量 44 kDa
GenBank蛋白编号BC007814
基因名称 HLA class I (HLA-C)
Gene ID (NCBI) 3107
RRIDAB_1639892
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP10321
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I (HLA-A, B and C) and class II (HLA-D). This class I molecules are polymorphic membrane glycoproteins composed of a heavy (alpha) chain (44 kDa) which is encoded by a HLA class I gene (HLA-A, B or C), and β2-microglobulin light (beta) chain (12 kDa). They are involved in the presentation of foreign antigens to the immune system. This polyclonal antibody raised against human HLA-C can also react with HLA-A and HLA-B. (PMID: 667938; 3375250)

实验方案

Product Specific Protocols
WB protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IHC protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IF protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
IP protocol for HLA class I (HLA-C) antibody 15777-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Matern Fetal Neonatal Med

HLA-C promotes proliferation and cell cycle progression in trophoblast cells

Authors - Hong Lv
View Article
humanIHC

Front Pharmacol

Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis

Authors - Yanling Wang
View Article
humanCell treatment

Nature

Shifts in receptors during submergence of an encephalitic arbovirus

Authors - Wanyu Li
View Article