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Anti-Human HLA-DR (L243) Rabbit Recombinant Antibody, PBS Only

HLA-DR Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC

Conjugate

Unconjugated

CloneNo.

L243

Cat No : 65503-1-PBS

Print datasheet

Synonyms

HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA

验证数据展示

  • FC experiment of human PBMCs using 65503-1-PBS

    FC experiment of human PBMCs using 65503-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone: L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

  • FC experiment of human PBMCs using 65503-1-PBS

    FC experiment of human PBMCs using 65503-1-PBS

    1x10^6 human PBMCs were surface stained with 0.1 ug Anti-Human HLA-DR Rabbit Recombinant Antibody (65503-1-PBS, Clone:L243) or Rabbit IgG control PolyAb and CoraLite®647-conjugated AffiniPure F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed. Lymphocytes and monocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65503-1-PBS targets HLA-DR in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen Human lymphoblastoid B-cell line RPMI 8866 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name major histocompatibility complex, class II, DR alpha
Synonyms HLA DRA, HLA DRA1, HLA-DRA, MHC class II antigen DRA
Calculated Molecular Weight 254 aa, 29 kDa
GenBank Accession NumberBC032350
Gene Symbol HLA-DRA
Gene ID (NCBI) 3122
ENSEMBL Gene IDENSG00000204287
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C. Stable for one year after shipment.

背景介绍

HLA-DR is a member of MHC class II moleculesencoded by the human leukocyte antigen complex on the region 6p21.31 of chromosome 6. HLA-DR is composed of two non-covalently associated transmembrane glycoproteins (the α and β chains), and is primarily expressed on B lymphocytes, monocytes, dendritic cells and thymic epithelial cells (PMID: 33125816). HLA-DR participate in the antigen presentation to CD4+ or CD8+ T cells and is postulated as a general biomarker of sepsis-induced immunosuppression (PMID: 28199206, 32421903). The Anti-Human HLA-DR antibody, clone L243, is derived from Human lymphoblastoid B-cell line RPMI 8866.

实验方案

Product Specific Protocols
FC protocol for HLA-DR antibody 65503-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols