HLA-DRB1 Polyclonal antibody
HLA-DRB1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human spleen tissue, A375 cells, Daudi cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy HLA-DRB1 Ready-To-Use IHC Kit. HLA-DRB1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
15862-1-AP targets HLA-DRB1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | HLA-DRB1 fusion protein Ag8405 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | major histocompatibility complex, class II, DR beta 1 |
| Synonyms | DR4, DRB1, DW2.2/DR2.2, HLA DR1B, HLA DRB, HLA DRB1, HLA DRB1*, HLA DRB2, HLA-DRB1, MHC class II antigen DRB1*15, SS1 |
| Calculated Molecular Weight | 266 aa, 30 kDa |
| Observed Molecular Weight | 27-30 kDa |
| GenBank Accession Number | BC007920 |
| Gene Symbol | HLA-DRB1 |
| Gene ID (NCBI) | 3123 |
| RRID | AB_1639897 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P01911 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| IHC protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| IP protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| FC protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation. | ||
Nat Commun Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes | ||
Heliyon Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation | ||
Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis |
