验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, human spleen tissue, A375 cells, Daudi cells |
| Positive IP detected in | Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Raji cells |
Planning an IHC experiment? We recommend our IHCeasy HLA-DRB1 Ready-To-Use IHC Kit. HLA-DRB1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 1 publications below |
产品信息
15862-1-AP targets HLA-DRB1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | HLA-DRB1 fusion protein Ag8405 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | major histocompatibility complex, class II, DR beta 1 |
| 别名 | Human leukocyte antigen DRB1, HLA DRB1, HLA DRB, HLA DR1B, HLA class II histocompatibility antigen, DRB1 beta chain |
| 计算分子量 | 266 aa, 30 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC007920 |
| 基因名称 | HLA-DRB1 |
| Gene ID (NCBI) | 3123 |
| RRID | AB_1639897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P01911 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| IHC protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| IF protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| IP protocol for HLA-DRB1 antibody 15862-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Pharmacol Inositol-C2-PAF down-regulates components of the antigen presentation machinery in a 2D-model of epidermal inflammation. | ||
Nat Commun Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes | ||
Heliyon Qingda granule prevents obesity-induced hypertension and cardiac dysfunction by inhibiting adverse Akt signaling activation | ||
Front Pharmacol Proteomics study the potential targets for Rifampicin-resistant spinal tuberculosis |
