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HLA-F Polyclonal antibody

HLA-F Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14670-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA and More (1)

HLA-5.4, HLA F antigen, HLA F, HLA CDA12, HLA 5.4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRaji cells, human placenta tissue, human spleen tissue, A431 cells
Positive IHC detected inhuman tonsillitis tissue, human placenta tissue, human skin cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14670-1-AP targets HLA-F in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 HLA-F fusion protein Ag6335 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 major histocompatibility complex, class I, F
别名 HLA-5.4, HLA F antigen, HLA F, HLA CDA12, HLA 5.4
计算分子量 39 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC062991
基因名称 HLA-F
Gene ID (NCBI) 3134
RRIDAB_1519280
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP30511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

实验方案

Product Specific Protocols
WB protocol for HLA-F antibody 14670-1-APDownload protocol
IHC protocol for HLA-F antibody 14670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratIHC

Kidney Int

Upregulation of HLA-F expression by BK polyomavirus infection induces immune recognition by KIR3DS1-positive natural killer cells.

Authors - Tobias F Koyro
humanIHC

Int J Cancer

Alteration of HLA-F and HLA I antigen expression in the tumor is associated with survival in patients with esophageal squamous cell carcinoma.

Authors - Zhang X X

J Immunol

Immune Modifications in Fetal Membranes Overlying the Cervix Precede Parturition in Humans.

Authors - Louis Marcellin
mouse,humanWB

Int J Biol Sci

Targeting HLA-F suppresses the proliferation of glioma cells via a reduction in hexokinase 2-dependent glycolysis.

Authors - Xin Chen
  • KD Validated
humanWB,IHC

Lung Cancer

HLA-F expression is a prognostic factor in patients with non-small-cell lung cancer.

Authors - Lin Aifen A
humanIHC

Oncol Lett

Lesion human leukocyte antigen-F expression is associated with a poor prognosis in patients with hepatocellular carcinoma.

Authors - Yongfu Xu
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